WebMolecular mechanism of tRNA binding by the Escherichia coli N7 guanosine methyltransferase TrmB Molecular mechanism of tRNA binding by the Escherichia coli N7 guanosine methyltransferase TrmB J Biol Chem. doi: 10.1016/j.jbc.2024.104612. Online ahead of print. Authors SarahK Schultz 1 , Kieran Meadows 2 , Ute Kothe 3 Affiliations WebSep 26, 2024 · We demonstrated that the P. aeruginosa trmB gene encodes a tRNA guanine (46)-N7-methyltransferase that catalyzes the formation of m 7 G46 in the tRNA variable …
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WebMar 16, 2024 · By comparing how tRNA interacts with wildtype TrmB compared to catalytically-impaired TrmB D144A, we can gain insight into the kinetic mechanism of … WebMay 15, 2008 · Aquifex aeolicus is a hyper thermophilic eubacterium that grows at close to 95 degrees C. A. aeolicus tRNA (m (7)G46) methyltransferase [TrmB] has an elongated C-terminal region as compared with mesophilic counterparts. In this study, the authors focused on the functions of this C-terminal region. how do you figure bmi index
trmB tRNA (guanosine (46)-N7)-methyltransferase TrmB []
WebTitle Check will be unavailable on Sunday, April 23, 2024, from 7:00 AM to 12:00 PM (CDT) to enable system maintenance. Thank you for your patience as we strive to provide the best … WebOct 30, 2024 · To gain insight into the molecular mechanism of tRNA binding by E. coli TrmB in real-time, we developed a new assay based on introducing a 4-thiouridine modification at position 8 of in vitro transcribed tRNA Phe enabling us to fluorescently labelled this unmodified tRNA. WebMar 3, 2024 · tRNA is the most extensively modified RNA in cells. On average, a bacterial tRNA contains 8 modifications per molecule and a eukaryotic tRNA contains 13 modifications per molecule. Recent studies reveal that tRNA modifications are highly dynamic and respond extensively to environmental conditions. phoenix md what county