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Hy5 interaction protein

Web11 feb. 2016 · HY5-YFP was detectable in the input and IP fractions (Fig. 1f and Additional file 1 d) although it ran slightly higher than its predicted molecular weight of 45 kDa (18 kDa HY5 plus 27 kDa YFP). However, a similar size discrepancy has been observed previously (endogenous HY5 migrating at 30 kDa) [ 26 ]. Web25 mei 2000 · HY5 is a bZIP transcription factor that binds directly to the promoters of light-inducible genes, promoting their expression and photomorphogenic development 2, 3. …

The COP1–SPA1 interaction defines a critical step in …

WebDET1 associates with COP1 in vivo, enhances COP1–HY5 interaction, and promotes COP1 destabilization in a process that dampens HY5 protein abundance. By restricting HY5 abundance, DET1 precludes HY5 binding to secondary targets and avoids hyper-photomorphogenic phenotypes in Arabidopsis. http://repository.ias.ac.in/112112/1/1036.full.pdf canisius golden griffins basketball https://davidsimko.com

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WebNational Center for Biotechnology Information WebIn this way, the deprivation of ubiquitin-mediated protein has turned out to be an essential theme in various plant hormone signaling pathways. Got a question on this topic? Find answers in our Expert Q&A. Search Search Search done loading. What you'll learn: Plant hormone signaling Definition; Web8 okt. 2024 · To further confirm the interaction of AGB1 with HY5 in plant cells, we performed BiFC assays and found that AGB1 interacted with HY5 in the nucleus (Figure 4 E).Since HY5 protein is degraded in the dark (Osterlund et al., 2000), we then performed Co-IP assays with light-grown transgenic Arabidopsis overexpressing FLAG-AGB1 in the … five little easter bunnies tayo

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Hy5 interaction protein

Arabidopsis chromatin remodeling factor PICKLE interacts with ...

Web1 sep. 2000 · To test interactions, the capacity of the phosphorylated GSTHY5 (generated by kinase treatment) and dephosphorylated GSTHY5 (generated by a successive lambda protein phosphatase treatment) to pull down bacterially expressed and radioactively labeled FLAG-tagged COP1 in an in vitro protein interaction assay (Ang et al., 1998) was … WebCOP1 HY5. Biochemical Activity. Biochemical Activity. An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange …

Hy5 interaction protein

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Web6 mei 2024 · As shown in Figure 1D, the HY5-GFP protein was precipitated by the anti-HDA15 antibody in ProHY5:HY5-GFP seedlings. Taken together, these results indicated … WebTo examine whether direct protein interaction between SPA1 and HY5 is involved in the SPA1-mediated control of HY5 level, we used a yeast two-hybrid assay. Full-length SPA1 fused with the LexA DNA-binding domain interacts with the GAL4 activation-domain fusion of HY5 . This interaction is greatly reduced by point ...

Web14 okt. 2024 · As a key promoter of visible light and UV-B photomorphogenesis, HY5 functions together with the B-box family transcription factors BBX20–22 that were … WebCell Press October 10, 2016. ELONGATED HYPOCOTYL5 (HY5), a member of the bZIP transcription factor family, inhibits hypocotyl growth and lateral root development, and promotes pigment accumulation in a light-dependent manner in Arabidopsis. Recent research on its role in different processes such as hormone, nutrient, abiotic stress …

WebABA-induced COP1 cytoplasmic localization increased HY5 and ABI5 protein levels in the nucleus, leading to increased expression of ABI5 target genes and ROS levels in seeds. Together, our results reveal that ABA-induced cytoplasmic translocation of COP1 activates the HY5-ABI5 pathway to promote the expression of ABA-responsive genes and the … WebCa21-Binding Protein Involved in Cryptochrome and Phytochrome Coaction Hongwei Guo, Todd Mockler, Hien Duong, Chentao Lin* Cryptochromes and phytochromes are the major photosensory receptors in plants and often regulate similar photomorphogenic responses. The molecular mechanisms underlying functional interactions of cryptochromes and phyto-

Web1 nov. 2003 · To examine whether direct protein interaction between SPA1 and HY5 is involved in the SPA1-mediated control of HY5 level, we used a yeast two-hybrid assay. Full-length SPA1 fused with the LexA DNA-binding domain interacts with the GAL4 activation-domain fusion of HY5 ( Fig. 2E ).

Web6 feb. 2024 · The HY5 protein was clearly degraded by Tm treatment, and this Tm-induced degradation of HY5 was significantly inhibited by MG132 treatment (Fig. 4F), indicating … canisius college men\u0027s ice hockeyWebAbstract. ELONGATED HYPOCOTYL5 (HY5) is a basic domain/leucine zipper (bZIP) transcription factor, central for the regulation of seedling photomorphogenesis. Here, we … five little ducks went swimming one day wordsWeb13 dec. 2024 · The BBX proteins were found to be involved in ABA signaling modulating plant growth and development. BBX21 represses the expression of ABI5 through physical interaction with HY5 or the ABI5 protein, therefore negatively regulating ABA-inhibited seed germination (Xu et al., 2014; Kang et al., 2024). five little easter rabbitsProtein Transcription factor HY5 Gene HY5 Status UniProtKB reviewed (Swiss-Prot) Organism Arabidopsis thaliana (Mouse-ear cress) Amino acids 168 Protein existence Evidence at protein level Annotation score 5/5 Entry Feature viewer Publications External links History BLAST Download Add … Meer weergeven canisius college writing centerWeb13 jul. 2024 · Here, the authors show that three B-box containing proteins (BBX) are cofactors of HY5 and modulate transcriptional responses for a subset of HY5-dependent … five little ducks storyWeb21 okt. 2024 · Karrikins control seedling photomorphogenesis and anthocyanin biosynthesis through a HY5-BBX transcriptional module. A Positive Feedback Loop of BBX11-BBX21-HY5 Promotes Photomorphogenic Development in Arabidopsis. Identification of BBX proteins as rate-limiting cofactors of HY5. interaction with HRB2 modulates ABI5 locus … five little easter bunniesWeb14 apr. 2024 · Soybean (Glycine max) is a major protein and oil crop. Soybean basic region/leucine zipper (bZIP) transcription factors are involved in many regulatory pathways, including yield, stress responses, environmental signaling, and carbon-nitrogen balance. Here, we discuss the members of the soybean bZIP family and their classification: 161 … canisius golden griffins basketball schedule