Fls2 antibody
Webthat the FLS2–R protein complex formation does not depend on the HPB tag, we immunoprecipitated RPM1::Myc, which does not contain any part of the HPB tag, from … WebRabbit polyclonal antibodies were produced by immunizing animals with a GST-fusion protein containing the C-terminal region of arabidopsis thaliana FLS2 (At5g46330). …
Fls2 antibody
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WebMar 12, 2014 · A well-known PRR is the Arabidopsis receptor kinase FLS2, which recognizes a conserved 22 amino acid N-terminal sequence of the bacterial flagellin protein (flg22). FLS2 contains an extracellular leucine-rich repeat (LRR) domain, a transmembrane domain, and a cytoplasmic kinase domain ( Chinchilla et al., 2006 ). WebMar 1, 2024 · After SDS PAGE and western blot, proteins were detected using α-FLS2, α-GFP, and α-BAK1 antibodies (Chinchilla et al, 2007). For co-immunoprecipitation (Co-IP) experiments of FLS2-GFP and BAK1 in Col-0 pFLS2::FLS2-GFP lines, proteins were extracted from 12-day-old seedlings upon treatment with mock, 1 μM flg22, or co …
http://www.abiocode.com/products/?type=detail&id=2984 WebAn antibody raised against AtFLS2 (AtFLS2) efficiently immunoprecipitated AtFLS2 from solubilized microsomal membranes of Col-0 WT seedlings and La-er cultured cells but not from fls2 seedlings...
WebAug 31, 2024 · We observe rapid S-acylation of FLS2 upon perception of its flg22 ligand in a BAK1 co-receptor dependent manner. Notably, S-acylation is essential for several aspects of FLS2-mediated early and... WebJul 25, 2024 · (l) Immunoblot of 29 transiently expressing FLS2-GFPs in KO1&2 using an anti-GFP antibody. (m) ROS burst produced by KO1&2 leaves transiently expressing 7 …
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WebBackground: FLS2 constitutes the pattern-recognition receptor (PPR) that determines the specific perception of flagellin (flg22), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). high protein low fat snacks bodybuildingWebOct 10, 2013 · Present in most higher plant species and critical for antibacterial immunity ( 1 ), flagellin-sensitive 2 (FLS2) is an LRR-RK and acts as the PRR for bacterial flagellin by recognizing the epitope flg22 ( 2 – 6 ). Direct recognition of flg22 by FLS2 is sufficient for inducing immune responses, establishing FLS2 as a flagellin receptor ( 7 ). how many browser safe colors are thereWebSalmonella virus Fels2 is a virus of the family Myoviridae, genus Felsduovirus. [1] [2] As a member of the group I of the Baltimore classification, Salmonella virus Fels2 is a dsDNA … how many brownies in a 8x8 panWebDec 29, 2009 · The plant PRR FLS2, a leucine-rich repeat-receptor kinase, recognizes bacterial flagellin and initiates immune signaling by association with another leucine-rich repeat-receptor-like kinase, BAK1. It remains … how many brothers make up the us band hansonWebFLS2 antibody (ABIN4966167). Validated for WB. Tested in Arabidopsis thaliana. Order online. English ... Comparison List Basket Phone: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected]. Home Antibodies ELISA Kits Primary Antibodies. Monoclonal Antibodies; Polyclonal Antibodies; Recombinant Antibodies ... high protein low fat sourcesWebIn summary, we show that FLS2 binds flg22 and determines the specificity of this interaction. These biochemical and functional characteristics identify FLS2 as the bona fide pattern-recognition receptor for flagellin. RESULTS Antibodies against the C Terminus of FLS2 Specifically Detect a Polypeptide of ∼175 kD in Arabidopsis high protein low fat meal prepWebThe leucine-rich repeat receptor kinase (LRR-RK) FLS2 is the receptor for the bacterial PAMP flagellin or its active epitope, represented by the peptide flg22 (1, 2). Binding of flg22 to FLS2 triggers heterodimerization with and phosphorylation of the LRR-RK BAK1 (3, 4). high protein low fat vegetarian meals